文献引用抗体：bs-0465R

Anti-NFKB p65 pAb | IHC

作者单位：四川大学华西口腔医院口腔疾病国家临床研究中心口腔疾病国家重点实验室

摘要：Skin photodamage, which is induced by ultraviolet (UV) radiation, is a prevalent cause of skin damage. In this study, a transdermal drug delivery system is developed for the topical treatment of skin photodamage, which is composed of tetrahedral framework nucleic acids (tFNAs) and lipoic acid (LA). The tFNAs-LA (TLA) nanocomposite exhibits excellent biocompatibility, as well as antioxidant, anti-apoptotic, and anti-inflammatory capabilities. tFNA, as a carrier, facilitates TLA for cell entry and skin penetration, while the loaded LA enhances the antioxidant and anti-inflammatory capabilities. In photodamaged human dermal fibroblast (HDF), TLA promotes proliferation and migration while inhibiting apoptosis activation and reactive oxygen species production. Moreover, TLA modulates apoptosis-related proteins and NF-κB signaling pathways, increasing cellular secretion while suppressing inflammatory responses in photodamaged HDF cells. In the in vivo experiment, topical application of TLA promotes tissue healing in photodamaged skin, and regulates the expression of inflammation and collagen-related proteins. It is suggested that the transdermal ability of TLA enables non-invasive therapy for skin photodamage, highlighting the potential of employing nucleic acid-based transdermal drug delivery systems for skin disease.

Anti-CD86 pAb | IF

作者单位：中国医学科学院和中国协和医科大学生物医学工程研究所天津生物材料研究重点实验室

RIPA Lysis Buffer

作者单位：中国科学院深圳先进技术研究院生物医药与生物技术研究所人体组织器官退化研究中心

摘要：Mesenchymal stem cell-derived exosomes (MSC-Exos) have shown great potential in the areas of bone regeneration and treatment of age-related diseases. Engineered exosomes can integrate multiple functional components to achieve optimal, targeted therapeutic effects. This study combined large-scale generation, bone-targeting modification, and miR-26a loading for exosome-mimetics (EMs) to construct a cell-free delivery system that promotes bone regeneration with good biocompatibility. EMs were fabricated through sequential extrusion of MSCs and reached a 15-fold production yield compared to conventional exosome secretion. Systemic injection of Asp8-EM/miR-26a in mouse models accelerated bone-defect healing and prevented osteoporosis. The underlying mechanism involves miR-26a targeting glycogen synthase kinase-3β (GSK-3β) to induce β-catenin accumulation, thus activating Wnt signaling pathway and promoting bone regeneration. This study provides a feasible and effective strategy for modifying EMs to enhance bone regeneration.

作者单位：西南大学化学化工学院，发光分析与分子传感教育部重点实验室

摘要：Screening effective luminophore is always one of the most essential concerns in highly sensitive electrochemiluminescence (ECL) bioanalysis. Here, a new nanoflower-like iridium(III) J-aggregate (T-Ir) was prepared via nonionic surfactant assisted reprecipitation technique, which possessed good reductive-oxidative ECL activity and performed a strong ECL emission around 626 nm at low potential (−1.10 V) in the presence of potassium persulfate. Thus, T-Ir was employed as a luminophore and good biocompatible matrix to load large amounts of capture antibodies (Ab₁). Meanwhile, gold nanoparticles capped cuprous oxide nanocubes (Cu₂O@Au) were used as the efficient quencher to label detection antibodies (Ab₂). While different dosages of carcinoembryonic antigen (CEA) were introduced via sandwich immunoreaction, the ECL intensity was significantly dropped due to electrochemiluminescence resonance energy transfer from energy donor (T-Ir) to the acceptor (Cu₂O@Au), achieving the highly sensitive detection of CEA. The proposed immunosensor showed a linear concentration range from 1 fg·mL⁻¹ to 80 ng·mL⁻¹ with a low limit of detection of 0.93 fg·mL⁻¹ (S/N = 3). It featured superior stability, selectivity, reproducibility and practicability. With the successful demonstration of hydrophilic iridium(III)-based nanomaterials, sensing strategies for various biomarkers detection in diseases diagnosis will flourish.